MABN2397
Anti-MYCBP2 Antibody, clone PM-8A9
clone PM-8A9, from mouse
Synonym(s):
E3 ubiquitin-protein ligase MYCBP2, Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
PM-8A9, monoclonal
species reactivity
human
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... MYCBP2(23077)
General description
E3 ubiquitin-protein ligase MYCBP2 (UniProt: O75592; also known as Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc, MYCBP2) is encoded by the MYCBP2 (also known as KIAA0916, PAM) gene (Gene ID: 23077) in human.MYCBP2 protein mediates the ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. It is ubiquitously expressed, but brain and thymus display exceptionally abundant expression. It is shown to co-localize with TSC1 and TSC2 along the neurites and in the growth cones. MYCBP2 may also function as a facilitator or regulator of transcriptional activation by MYC and have a role during synaptogenesis. It is reported to negatively regulate neuronal growth, synaptogenesis, and synaptic strength. Mice with MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 is also linked to constitutively activated p38 MAPK and increased expression of several proteins involved in receptor trafficking. Down-regulation of MYCBP2 in the spinal cord of adult rats is shown to enhance their. MYCBP2 is also shown to be an inhibitor of adenylyl cyclase. (Ref.: Holland, S et al. (2011). J. Biol. Chem. 286(5): 3671-3680).
Specificity
Clone PM-8A9 specifically detects MYCBP2 in HEK293 cells. It targets an epitope within the first 150 amino acids in the N-terminal region.
Immunogen
Epitope: N-terminus
MBP (Maltose-binding protein)-tagged recombinat fragment of 150 amino acids from the N-terminal region of human MYCBP2.
Application
Anti-MYCBP2 Antibody, clone PM-8A9, Cat. No. MABN2397, is a highly specific mouse monoclonal antibody that targets MYCBP2 (PAM) and has been tested in Immunoprecipitation and Western blotting.
Immunoprecipitation Analysis: A representative lot detected MYCBP2 (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).
Western Blotting Analysis: A representative lot detected endogenous PAM (MYCBP2)and overexpressed Myc-tagged PAM (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).
Western Blotting Analysis: A representative lot detected endogenous PAM (MYCBP2)and overexpressed Myc-tagged PAM (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).
Research Category
Neuroscience
Neuroscience
Quality
Evaluated by Western Blotting in HEK293 cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MYCBP2 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MYCBP2 in 10 µg of HEK293 cell lysate.
Target description
~460 kDa observed; 510.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
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