480716

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

• Synonym(s): α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, N-Acetylneuraminyl Hydrolase, Sialidase
• CAS Number: 9001-67-6
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: liquid
• specific activity: ≥40 units/mg protein; ≥5 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).

Physical form

In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable