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Key Documents

1.10549

Millipore

Granucult® Listeria Enrichment Broth

According to FDA/IDF-FIL; For microbiology

Synonym(s):

Listeria Enrichment broth, LEB

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74
Pricing and availability is not currently available.

reg. compliance

FDA IDF-FIL

Quality Level

200

sterility

non-sterile

form

granular

manufacturer/tradename

GranuCult® plus

technique(s)

microbiological culture: suitable

pH

7.1-7.5 (30 °C, 36.1 g/L in H2O, after autoclaving)

solubility

36.1 g/L

application(s)

food and beverages
microbiology

storage temp.

2-30°C

suitability

Listeria spp.

General description

The Granucult® Listeria Enrichment Broth formulation is a modified Tryptic Soy (CASO) Broth with an additional 6 g/liter of yeast extract. The inhibition of undesired accompanying flora is achieved by the addition of acriflavin HCl, cycloheximide, and nalidixic acid. In contrary to Listeria Enrichment Broth (Base) (1.11951), this broth already contains antibiotic substances.

Application

Granucult® Listeria Enrichment Broth is intended for the selective enrichment of Listeria.

Linkage

Replaces: 1.10549.5007

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.1 - 7.5
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19114 (WDCM 00110)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 10 - 100
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)): 10 - 100
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 19114 (WDCM 00110)): ≥ 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 1E+04 CFU/ml
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 1E+04 CFU/ml
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≤ 1000 CFU/ml
Incubation: 24 hrs.; 30 °C; aerobically.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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