The American journal of physiology, 261(5 Pt 1), C906-C915 (1991-11-01)
Organotin derivatives represent a class of artificial ionophores that mediate Cl(-)-OH- exchange and thereby facilitate the chemical equilibrium distribution of Cl- and H+ across biological membranes. Imposing different pH and Cl- gradients by varying extracellular pH (pHo) and extracellular [Cl-]
Journal of cell science, 98 ( Pt 3), 281-291 (1991-03-01)
Different agents have been employed to extract the histones and other soluble components from isolated HeLa S3 nuclei during nuclear matrix isolation. We report that 0.2M (NH4)2SO4 is a milder extracting agent than NaCl and LIS (lithium 3,5-diiodosalicylate), on the
The Journal of general physiology, 100(5), 825-846 (1992-11-01)
We have developed a new test to differentiate between ping-pong and simultaneous mechanisms for tightly coupled anion exchange. This test requires the use of a dead-end reversible noncompetitive inhibitor. As an example, we have applied the test to the anion
Nuclear frameworks: concepts and operational definitions.
N Stuurman et al.
Cell biology international reports, 16(8), 837-852 (1992-08-01)
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(10), 965-970 (2008-08-02)
Rapid flow cytometric measurement of the frequency of aneuploid human sperms is in increasing demand but development of an exploitable method is hindered by difficulties of stoichiometric staining of sperm DNA. An aggressive decondensation protocol is needed after which cell
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