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Key Documents

201464

Sigma-Aldrich

Variamine Blue RT Salt

Synonym(s):

4-Aminodiphenylamine diazonium sulfate, 4-Diazodiphenylamine sulfate, Azoic Diazo Component 22, Azoic Diazo No. 22

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About This Item

Linear Formula:
C6H5NHC6H4N2 · HSO4
CAS Number:
Molecular Weight:
293.30
Colour Index Number:
37240
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

solid

solubility

1 M HCl: 10 mg/mL

λmax

377 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OS([O-])(=O)=O.N#[N+]c1ccc(Nc2ccccc2)cc1

InChI

1S/C12H10N3.H2O4S/c13-15-12-8-6-11(7-9-12)14-10-4-2-1-3-5-10;1-5(2,3)4/h1-9,14H;(H2,1,2,3,4)/q+1;/p-1

InChI key

FMRQRWPEQSPSDG-UHFFFAOYSA-M

Application

Variamine Blue RT Salt has been used as a dye in ultraviolet-visible spectrophotometric method based-uricase enzyme for the detection of uric acid in normal urine. It is also used in combination with naphthol AS-MX phosphate-variam to determine the cytochemical visualization of alkaline phosphatase activity.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Localization Mechanisms in Enzyme Cytochemistry
Studied with Alkaline Phosphatase-loaded
Erythrocyte Ghosts?
RAAP A K
The Journal of Histochemistry and Cytochemistry, 32, 815-820 (1984)

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