SAE0194

3xFLAG^™ Peptide

≥95% (HPLC), lyophilized powder, purified

• Synonym(s): Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide
• UNSPSC Code: 12352202
• NACRES: NA.43

Properties

• Product Name: Purified 3xFLAG^® peptide, ≥95% (HPLC), lyophilized powder
• biological source: synthetic
• Quality Level: 200
• Assay: ≥95% (HPLC)
• form: lyophilized powder
• technique(s): protein extraction: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residues in length. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution by affinity chromatography of 3X FLAG fusion proteins either from the ANTI-FLAG M2 monoclonal antibody (F1804) in solution, or bound to agarose on the ANTI-FLAG M2 agarose affinity gel (A2220).
For elution under gentle, mild conditions of 3X FLAG fusion proteins from the ANTI-FLAG M2 affinity gels.

(Note: the 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG affinity gels.)

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable