Skip to Content
Merck
All Photos(6)

Documents

RDD008

Sigma-Aldrich

Trizma® base

≥99.9%, powder, anhydrous, Redi-Dri

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

Sign Into View Organizational & Contract Pricing


About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product name

Trizma® base, anhydrous, free-flowing, Redi-Dri, ≥99.9%

grade

anhydrous

Quality Level

description

aminopeptidase substrate

product line

Redi-Dri

Assay

≥99.9%

form

powder

quality

free-flowing

does not contain

Carbon Fiber

impurities

≤0.2% Water (Karl Fischer)

color

white

pH

10.5-12
7-9

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (Water < 0.2%)

absorption

≤0.05 at 290 nm at 40%

λ

(A290 UV absorbance < 0.05)
in 5 mM Tris; DNA (40% (w/w))

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
  • To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
  • In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
  • In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
  • As a component of nuclei extraction buffer

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Top-down characterization of chicken core histones.
Wu H, et al.
Journal of Proteomics, 184, 34-38 (2018)
Extracellular protease digestion to evaluate membrane protein cell surface localization.
Besingi RN and Clark PL
Nature Protocols, 10(12), 2074-2074 (2015)
A Streamlined Method for the Preparation of Growth Factor-enriched Thermosensitive Hydrogels from Soft Tissue.
Poon CJ, et al.
Acta Biomaterialia (2013)
Active Cdk5 Immunoprecipitation and Kinase Assay.
Bankston AN, et al.
Bio-protocol, 7(13) (2017)
Preparation of Buffers for Use in Enzyme Studies.
Gomori G
Methods in Enzymology, 1, 138-146 (1955)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service