P3391
Protein A-Sepharose™ from Staphylococcus aureus
lyophilized powder
Synonym(s):
Protein A–Agarose
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About This Item
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form
lyophilized powder
packaging
glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g
storage condition
do not freeze
extent of labeling
3 mg per mL
technique(s)
affinity chromatography: suitable
color
white to gray
matrix
Sepharose CL-4B
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥16 mg/mL binding capacity (human IgG)
solubility
water: 1 g/L at 20 °C
suitability
conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
storage temp.
2-8°C
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General description
Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.
Application
Protein A-Sepharose was used:
- for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
- in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
- as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
- in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Quantity
Swelling: 1 g swells to approx. 4 ml.
Physical form
Supplied as lyophilized powder stabilized with lactose and dextran.
Legal Information
Sepharose is a trademark of Cytiva
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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