M9901
Mutanolysin from Streptomyces globisporus ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Synonym(s):
Mutanolysin
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About This Item
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biological source
bacterial (Streptomyces globisporus)
Quality Level
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
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General description
Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.
Application
Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Biochem/physiol Actions
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
Unit Definition
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
Physical form
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Fiberfrax is a registered trademark of Unifrax Corp.
substrate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci
Kondo, J. and L. McKay
Journal of Dairy Science, 65, 1428-1431 (1982)
G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
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