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L9379

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder

Synonym(s):

PWM, Pokeweed mitogen

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

sterility

aseptically filled

Quality Level

form

lyophilized powder

potency

≤0.3 μg/mL mitogenic activity

storage temp.

2-8°C

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General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Lectin from Phytolacca americana (pokeweed) has been used:
  • as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation
  • as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes
  • as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Physical form

Partially purified TCA precipitate.
Essentially salt-free.

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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