Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides
L4516
Lipopolysaccharides from Escherichia coli O127:B8
BioXtra, suitable for cell culture, γ-irradiated
Synonym(s):
LPS
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About This Item
Pricing and availability is not currently available.
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biological source
Escherichia coli (O127:B8)
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
purified by
gel-filtration chromatography
technique(s)
cell culture | mammalian: suitable
impurities
<1% Protein (Lowry)
solubility
H2O: 5 mg/mL, slightly hazy
storage temp.
2-8°C
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Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli 0127:B8 has been used:
- to study the LPS-induced macrophage survival in mice[1]
- to elicit the secretion of cytokines by cattle PBMC[2]
- to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment[1]
- to perform autophagy assay in BV2 and BV2-LC3 cells[3]
- for stimulation of human monocytes, macrophages and mouse microglia cells[4][5][6][7]
- to induce pentraxin-3 expression in 293T cells[8]
Biochem/physiol Actions
Reconstitution
Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Once reconstituted what is the stability at -20C for the LPS product L4516-1M?
1 answer-
Helpful?
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Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.
1 answer-
LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.See Sigmacote, SL2:
https://www.sigmaaldrich.com/product/sigma/sl2For additional information, see the link below to review the Lipopolysaccharides product information page:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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