Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
L4516
Lipopolysaccharides from Escherichia coli O127:B8
BioXtra, suitable for cell culture, γ-irradiated
Synonym(s):
LPS
About This Item
Recommended Products
biological source
Escherichia coli (O127:B8)
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
purified by
gel-filtration chromatography
technique(s)
cell culture | mammalian: suitable
impurities
<1% Protein (Lowry)
solubility
H2O: 5 mg/mL, slightly hazy
storage temp.
2-8°C
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Application
- to study the LPS-induced macrophage survival in mice[1]
- to elicit the secretion of cytokines by cattle PBMC[2]
- to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment[1]
- to perform autophagy assay in BV2 and BV2-LC3 cells[3]
- for stimulation of human monocytes, macrophages and mouse microglia cells[4][5][6][7]
- to induce pentraxin-3 expression in 293T cells[8]
Biochem/physiol Actions
Reconstitution
Other Notes
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Customers Also Viewed
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Once reconstituted what is the stability at -20C for the LPS product L4516-1M?
1 answer-
Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.
1 answer-
LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.See Sigmacote, SL2:
https://www.sigmaaldrich.com/product/sigma/sl2For additional information, see the link below to review the Lipopolysaccharides product information page:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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