G5507
Galactosyltransferase from bovine milk
lyophilized powder
Synonym(s):
Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
lyophilized powder
Quality Level
specific activity
3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)
composition
Protein, 35-65% modified Warburg-Christian
impurities
≤10 % protein α-lactalbumin
storage temp.
−20°C
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General description
Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.
Application
Galactosyltransferase from bovine milk has been used:
- in the generation of hyper-galactosylated variant of IgG1
- in β -1,4-galactosyltransferase assay rice root cell extracts
- in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers
Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.
Biochem/physiol Actions
Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various disease states.Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.
Linkage
This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.
Unit Definition
One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)
Physical form
Lyophilized powder containing Tris, EDTA, and (NH4)2SO4
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The kinetic mechansim of bovine milk galactosyltransferase. The role of alpha-lactalbumin.
Bell JE, et al.
The Journal of Biological Chemistry, 251(10), 3003-3013 (1976)
V Kolb et al.
Glycoconjugate journal, 16(9), 537-544 (2000-05-18)
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H Narimatsu et al.
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