FD2000S
Fluorescein isothiocyanate–dextran
average mol wt 2,000,000
Synonym(s):
FITC–Dextran
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About This Item
Recommended Products
biological source
synthetic (organic)
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 2,000,000
extent of labeling
0.003-0.020 mol FITC per mol glucose
color
yellow to orange
solubility
H2O: soluble 25 mg/mL
storage temp.
2-8°C
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General description
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
Fluorescein-dextran, 2000 kDa, was used for in vivo imaging of brain microvasculature in active mice using a minature integrated fluorecent microscope. FITC-dextran, 2000 kDa (FD-2000) is used in cardiovascular, microcirculation, perfusion and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Fluorescein-dextran, 2000 kDa, may be used for in vivo imaging of brain microvasculature in active mice using a miniature integrated fluorescent microscope. FITC-dextran has been used to study plant cell wall porosity.
Preparation Note
Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.
Other Notes
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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