C0992
Anti-Cy3/Cy5 antibody, Mouse monoclonal
clone CY-96, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Cy3/Cy5, Cy3 Antibody, Cy3 Antibody - Anti-Cy3/Cy5 antibody, Mouse monoclonal
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CY-96, monoclonal
form
buffered aqueous solution
concentration
~1.5 mg/mL
technique(s)
direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.
Specificity
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
Immunogen
mixture of proteins labeled with Cy3/Cy5.
Application
Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:
- immunofluorescence
- western blot
- dot blot
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- protein microarrays
- In in situ hybridization
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Biochem/physiol Actions
Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Pier Giorgio Righetti et al.
European journal of mass spectrometry (Chichester, England), 10(3), 335-348 (2004-06-10)
The present review attempts to cover the vast array of methods which have appeared in the last few years for performing quantitative proteome analysis. These methods are divided into two classes: those applicable to conventional two-dimensional map analysis, coupling orthogonally
Chiaki Nagai-Okatani et al.
International journal of molecular sciences, 20(3) (2019-02-10)
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method for cell type-selective glycomic profiling of tissue fragments
Te Liu et al.
Molecular medicine reports, 13(6), 5053-5058 (2016-04-29)
Premature ovarian failure (POF) is a common cause of female infertility, for which there are currently no ideal treatments or medications. Furthermore, apoptosis of ovarian granulosa cells (OGCs) is an important mechanism underlying the decline in ovarian reserve and function.
Wonbae Lee et al.
Nucleic acids research, 42(9), 5967-5977 (2014-03-15)
DNA constructs labeled with cyanine fluorescent dyes are important substrates for single-molecule (sm) studies of the functional activity of protein-DNA complexes. We previously studied the local DNA backbone fluctuations of replication fork and primer-template DNA constructs labeled with Cy3/Cy5 donor-acceptor
Myosin-Va mediates RNA distribution in primary fibroblasts from multiple organs
Salerno V P, et al.
Cell Motility and the Cytoskeleton, 65(5), 422-433 (2008)
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