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85558

Sigma-Aldrich

Spermidine

BioUltra, for molecular biology, ≥99.5% (GC)

Synonym(s):

SPD, 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane

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About This Item

Linear Formula:
NH2(CH2)3NH(CH2)4NH2
CAS Number:
Molecular Weight:
145.25
Beilstein:
1698591
EC Number:
MDL number:
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.26

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.5% (GC)

storage condition

(Tightly closed. Keep locked up or in an area accessible only to qualified or authorizedpersons.)

technique(s)

ChIP: suitable
buffer exchange: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.479 (lit.)

pH

12.0-13.5 (25 °C, 1 M in H2O)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

0.925 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.05

SMILES string

NCCCCNCCCN

suitability

suitable for molecular biology

application(s)

advanced drug delivery
genomic analysis

InChI

1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2

InChI key

ATHGHQPFGPMSJY-UHFFFAOYSA-N

Gene Information

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General description

Spermidine is a polyamine and a trivalent organic cation.Spermidine is a naturally occurring polyamine compound with diverse biochemical properties. It plays a vital role in gene expression, participating in DNA replication, transcription, and translation processes. Spermidine is crucial for cell growth, proliferation, and differentiation, influencing cell cycle progression and tissue development. It possesses antioxidant activity, safeguarding cells against oxidative stress by scavenging free radicals. Spermidine also stimulates autophagy, promoting the turnover of damaged organelles and protein aggregates for cellular homeostasis. With neuroprotective effects, it supports neuronal survival, synaptic plasticity, and guards against neurodegenerative diseases. Additionally, spermidine modulates cellular signaling pathways involved in inflammation and immune responses.

Application

Spermidine has been used:
  • as a component of chromosome isolation buffer
  • as a component of transcription buffer
  • as a component of homogenization buffer

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Spermidine modulates various cellular activities like cellular growth and development, stability of DNA and apoptosis.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Molecular Biology and Biochemical research
  • Free from DNase, RNase, NICKase, and protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Tested for trace levels of Anions and Cations

Caution

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Analysis Note

liquid or solid

Other Notes

For additional information on our range of Biochemicals, please complete this form.
In first strand cDNA synthesis; Effect on endonuclease inhibition by agarose contaminants

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

233.6 °F - closed cup

Flash Point(C)

112 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) on chromosomes in suspension for analysis of repetitive DNA by flow cytometry
Brind AJ and Lansdorp PM
Protocol Exchange (2011)
Biochemical Isolation of Myonuclei from Mouse Skeletal Muscle Tissue
Cutler AA, et al.
Bio-protocol, 7(24) (2017)
Restriction Endonucleases and Modification Methylases
Enzymology Primer for Recombinant DNA Technology, 233-306 (1996)
Induction of Autophagy and Apoptosis in Melanoma Treated With Palladacycle Complexes
Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging, 231-247 (2016)
Foundations of identifying individual chromosomes by imaging flow cytometry with applications in radiation biodosimetry
Beaton-GLA, et al.
Methods, 18-24 (2017)

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