54457
HEPES
≥99.5% (titration), powder, for molecular biology, BioUltra
Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
About This Item
Recommended Products
product name
HEPES, BioUltra, for molecular biology, ≥99.5% (T)
grade
for molecular biology
Quality Level
product line
BioUltra
Assay
≥99.5% (T)
form
powder
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
ign. residue
≤0.1% (as SO4)
loss
≤0.5% loss on drying, 110 °C
pH
5.0-6.5 (25 °C, 1 M in H2O)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
H2O: 1 M at 20 °C, clear, colorless
anion traces
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
λ
1 M in H2O
UV absorption
λ: 260 nm Amax: 0.045
λ: 280 nm Amax: 0.035
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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General description
Application
- DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
- RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
- Recapture solution to re-capture RNA exosomes from the glycerol gradient
- ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
- 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors
Other Notes
comparable product
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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