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31149

Sigma-Aldrich

Deoxyribonucleic acid, low molecular weight from salmon sperm

Synonym(s):

Salmon sperm dsDNA, low molecular weight DNA, DNA

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology
puriss. p.a.

form

lyophilized powder

impurities

≤5% protein

solubility

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

absorbance ratio

A260/280 nm ~1.4

storage temp.

2-8°C

Application

Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
  • to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.
  • in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.
  • as a precursor in the synthesis of the bio-dots.

Biochem/physiol Actions

Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David Czeiger et al.
American journal of clinical pathology, 135(2), 264-270 (2011-01-14)
Elevated circulating cell-free DNA (CFD) levels were found in patients with cancer. The standard CFD assays are work-intensive and expensive. The aim was to evaluate in patients with cancer a new simple CFD assay. In mice inoculated with cancer cells
Jing Yu et al.
Molecular biology of the cell, 13(2), 558-569 (2002-02-21)
Dramatic changes occur in nuclear organization and function during the critical developmental transition from meiosis to mitosis. The Drosophila nuclear lamina protein YA binds to chromatin and is uniquely required for this transition. In this study, we dissected YA's binding
P K Ganguli et al.
European journal of biochemistry, 101(2), 377-383 (1979-11-01)
Two native DNAs differing in G + C content were bound equally with the antitumour drug cis-Pt(NH3)2Cl2 at increasing Pt/P ratios. Resulting changes in their ultraviolet absorption spectra show equal fractional decreases in the initially different values of A250/A270 for
Qiuxia Chen et al.
SLAS discovery : advancing life sciences R & D, 25(5), 523-529 (2019-12-21)
DNA-encoded library (DEL) technology has been used as an ultra-high-throughput screening approach for hit identification of drug targets. This process is an affinity-based selection and requires incubation of DEL molecules with the target. Currently, in most reported cases, the input
Andrea Hájková et al.
Bioelectrochemistry (Amsterdam, Netherlands), 116, 1-9 (2017-03-18)
A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual

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