25668

Chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

• Synonym(s): Chloroform:Isoamyl Alcohol Solvent Mix
• MDL number: MFCD00171265
• UNSPSC Code: 12352401
• PubChem Substance ID: 329752812
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• Assay: ≥99.5% (chloroform + isoamyl alcohol, GC)
• form: liquid
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.001% free alkali (as NH₃)
• cation traces: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.02 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤0.5 mg/kg; Ni: ≤0.02 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.1; λ: 280 nm A_max: 0.07
• SMILES string: ClC(Cl)Cl.CC(C)CCO
• InChI: 1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H
• InChI key: BKHZIBWEHPHYAI-UHFFFAOYSA-N

Description

Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Components

mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302,H315,H319,H331,H336,H351,H361d,H372
• Precautionary Statements: P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
• Target Organs: Central nervous system, Liver,Kidney
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves