HPPIKRO
Roche
High Pure Plasmid Isolation Kit
Roche, pkg of 50 purifications (11754777001), pkg of 250 purifications (11754785001)
Sign Into View Organizational & Contract Pricing
All Photos(4)
About This Item
Recommended Products
manufacturer/tradename
Roche
Quality Level
packaging
pkg of 250 purifications (11754785001)
pkg of 50 purifications (11754777001)
General description
Low to medium throughput, mini scale, plasmid isolation.
The High Pure Plasmid Isolation Kit isolates purified plasmid DNA in small quantities using the alkaline lysis method, a commonly used method that generates highly purified plasmid DNA from E. coli, free of RNA contamination.
The High Pure Plasmid Isolation Kit isolates purified plasmid DNA in small quantities using the alkaline lysis method, a commonly used method that generates highly purified plasmid DNA from E. coli, free of RNA contamination.
Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.
Sample: 0.5 - 4.0 mL cultures of E. coli (harvested at a density of 1.5 - 5.0 A 600 units/mL)
Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.
Sample: 0.5 - 4.0 mL cultures of E. coli (harvested at a density of 1.5 - 5.0 A 600 units/mL)
Application
The High Pure Plasmid Isolation Kit prepares up to 15 μg purified plasmid DNA from bacterial cultures, that can be used directly in most molecular biology applications:
- PCR
- Sequencing
- Cloning
- In vitro transcription
- Restriction enzyme digests
- Random primed labeling
Features and Benefits
- Quickly purify up to 24 plasmid samples in <30 minutes.
- Minimize DNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
- Improve reliability and reproducibility in downstream applications with a kit that removes RNA and other impurities that cause plasmid DNA to behave unpredictably.
- Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.
Components
- Suspension Buffer
- RNase A, dry powder
- Lysis Buffer
- Binding Buffer
- Wash Buffer I
- Wash Buffer II
- Elution Buffer
- High Pure Spin Filter Tubes (containing glass fiber fleece)
- Collection Tubes
Quality
Plasmid pUC19 (4.5 μg) is purified from a 1.5 mL suspension of E. coli JM83, which was grown in LB-medium with ampicillin for 16 hours, to a cell density of 5 A 600 units/mL. 1 μg of the purified plasmid DNA is incubated for 1 hour at +37°C with 5 units of the restriction endonuclease Eco RI and then analyzed by agarose gel electrophoresis. The isolated plasmid DNA is as sensitive to restriction endonuclease digestion as plasmid DNA isolated by CsCl density centrifugation.
Preparation Note
The kit relies on alkaline lysis to release plasmid DNA from bacteria. RNase removes all RNA in the lysate. After cellular debris and (entrapped) genomic DNA are removed by centrifugation, the remaining supernatant is mixed with a chaotropic salt and applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, the plasmid binds to the glass fiber fleece, while contaminating substances (salts, proteins, and other cellular contaminants) do not. Brief wash-and-spin steps readily remove these contaminants. Once purified, the plasmid can be easily eluted in a small volume of low-salt buffer or water.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Corr. 1
Storage Class Code
8B - Non-combustible, corrosive hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Journal of clinical periodontology, 38(6), 581-589 (2011-04-15)
Recent studies showed that qPCR could detect bacteria related to periodontitis and peri-implantitis in a low concentration after full-mouth tooth extraction. This study monitored the microbiota from tooth extraction, over 9 months of full edentulism, up to 1 year after
Function Based Metagenomics Approach for Obtaining Lipase from Acid Mine Drainage Site.
International Journal Series in Engineering Science, 1-20 (2017)
Chromosome evolution in Solanum traced by cross-species BAC-FISH.
The New phytologist, 195(3), 688-698 (2012)
Molecular, cytological, and immunocytochemical study and kDNA
sequencing of laryngeal Leishmania infantum infectionv
sequencing of laryngeal Leishmania infantum infectionv
Parasitology Research, 112(4), 1799-1804 (2013)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service