11767291910

TUNEL Label Mix

sufficient for 30 tests, pkg of 3 × 550 μL

• Synonym(s): transferase dUTP nick end labeling, tunel
• UNSPSC Code: 41105500

Properties

• form: solution
• Quality Level: 100
• usage: sufficient for 30 tests
• packaging: pkg of 3 × 550 μL
• manufacturer/tradename: Roche
• color: colorless
• solubility: water: miscible
• storage temp.: −20°C

Description

General description

The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.

Application

TUNEL Label Mix has been used for the determination of apoptosis using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and DNA nick-end labeling by the TUNEL method.

The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL Enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.

Preparation Note

Working solution: TUNEL Label is used in combination with the TUNEL Enzyme to prepare the TUNEL reaction mixture.
For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H350i,H411
• Precautionary Statements: P201 - P273 - P280 - P308 + P313 - P391 - P501
• Hazard Classifications: Aquatic Chronic 2 - Carc. 1B Inhalation
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash