MAB1285
Anti-HLA-B27 Antibody, clone HLA.ABC.m3
clone HLA.ABC.m3, Chemicon®, from mouse
Synonym(s):
Anti-AS, Anti-B-4901, Anti-HLAB
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
HLA.ABC.m3, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG2a
NCBI accession no.
General description
Specificity
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
Application
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
Physical form
Storage and Stability
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Legal Information
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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