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MAB1285

Sigma-Aldrich

Anti-HLA-B27 Antibody, clone HLA.ABC.m3

clone HLA.ABC.m3, Chemicon®, from mouse

Synonym(s):

Anti-AS, Anti-B-4901, Anti-HLAB

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

HLA.ABC.m3, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG2a

NCBI accession no.

General description

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Specificity

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

Cell reactivity:

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:



Subjects Fluorescence Intensity

Heterozygous HLA-B27+ Strong

Homozygous HLA-B7+ Strong

Heterozygous HLA-B7+ Faint

Non B27, Non B7 Negative

Application

This Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

2. Cytotoxicity testing 1:25.

Physical form

Format: Purified
The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

Storage and Stability

Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shengli Song et al.
Frontiers in immunology, 12, 705140-705140 (2021-07-31)
Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major histocompatibility complex (MHC) mismatches are especially problematic. Most research directed
Barbara Neerinckx et al.
RMD open, 3(1), e000451-e000451 (2017-09-08)
The strong genetic association between HLA-B27 and ankylosing spondylitis has been known for over 40 years. HLA-B27 positivity is possibly associated with severity of ankylosis. We studied the in vitro and in vivo impact of HLA-B27 in models of chondrogenesis
Christoffer Dellgren et al.
Journal of immunology (Baltimore, Md. : 1950), 197(12), 4807-4816 (2016-11-09)
HLA class I cell surface expression is crucial for normal immune responses, and variability in HLA expression may influence the course of infections. We have previously shown that classical HLA class I expression on many human cell types is biased
Congjing An et al.
Cancer cell international, 22(1), 184-184 (2022-05-14)
Breast cancer (BC) is one of the most prevalent malignancies among women globally. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are associated with BC carcinogenesis. In the current study, we explored the mechanism by which LINC00662 regulates BC. Quantitative

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