Skip to Content
Merck
All Photos(1)

Key Documents

56401AST

Supelco

apHera NH₂ (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Amino HPLC Column

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

apHera NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L82

product line

apHera

packaging

pkg of 1 ea

manufacturer/tradename

apHera

parameter

147 bar pressure (2133 psi)
4-50 °C temperature

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

matrix

polymeric

matrix active group

amino phase

particle size

5 μm

pore size

300 Å

operating pH

1-14

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

Looking for similar products? Visit Product Comparison Guide

General description

apHera amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera Columns
  • High efficiency for carbohydrate analysis
  • Ideal for basic conditions/amphoteric detection
  • High selectivity mono- to oligosaccharides
  • pH range 2-13
  • 300 angstrom pore size
  • Stable, predictable retention
  • Long column life

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in

Articles

The apHera NH2 polymeric column is an excellent choice for the separation of closely-related polar analytes such as simple sugars using HILIC chromatography.

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service