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SAB4301419

Sigma-Aldrich

Anti-phospho-NF-κB p100 (pSer872) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Lyt10, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

100 kDa

species reactivity

rat, human, mouse

concentration

1.0 mg/mL

technique(s)

western blot: 1:500-1:1000 (Cell Lysate)

isotype

IgG

accession no.

NP_001070962.1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer872)

Gene Information

human ... NFKB2(4791)

General description

Nuclear factor kappa B subunit 2 (NFKB2) encodes the nuclear factor kappa B2 (NF-κB), a nuclear transcription factor. NF-κB is evolutionarily highly conserved. In human chromosome, the gene NFKB2 is localized on 10q24.

Specificity

The antibody detects endogenous level of NF-κB p100 only when phosphorylated at serine 872.

Immunogen

Peptide sequence around phosphorylation site of serine872(S-Q-S(p)-V-E) derived from Human NF-κB p100 .

Biochem/physiol Actions

Regulation of nuclear factor kappa B subunit 2 (NFKB2) includes cytoplasmic-nuclear shuttling and its transcriptional activity. NF-κB plays a key role in regulating the immune response and apoptotic processes. It is necessary for combating diseases like arthritis, autoimmune diseases and asthma. Mutations in NFKB2 impairs post translational processing of p100 preventing nuclear translocation of p52. This leads to common variable immunodeficiency and adrenal insufficiency. Mutation also affects canonical and non-canonical pathway of NFKB2 signalling and causes autosomal dominant primary antibody deficiency (PAD). chromosomal aberrations in C-terminal region of NFKB2 is associated lymphoid malignancies.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Missing pieces in the NF-kappaB puzzle
Ghosh S and Karin M
Cell, 109(2), S81-S96 (2002)
Analysis of NFKB2-mediated regulation of mechanisms underlying the development of Hodgkin's lymphoma
Zhang Y, et al.
Molecular Medicine Reports, 17(6), 8129-8136 (2018)
Mutations in NFKB2 and potential genetic heterogeneity in patients with DAVID syndrome, having variable endocrine and immune deficiencies
Brue T, et al.
BMC Medical Genetics, 15(1), 139-139 (2014)
Germline mutations in NFKB2 implicate the noncanonical NF-?B pathway in the pathogenesis of common variable immunodeficiency.
Chen K
American Journal of Human Genetics, 93(5), 812-824 (2013)
Autosomal dominant B cell deficiency with alopecia due to a mutation in NFKB2 that results in non-processible p100
Lee CE, et al.
Blood, 124(5), 2964-2972 (2014)

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