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SAB4200210

Sigma-Aldrich

Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-CPLA2-alpha, Anti-Group IVA, Anti-PLA2G4A PLA2G4, Anti-cytosolic phospholipase A2

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

western blot: 2-4 μg/mL using extracts of HEK-293T cells over expressing human cPLA2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... cPLA2(5321)

General description

Cytosolic phospholipase A2, group IVa, (cPLA2, also known as cPLA2a, PLA2G4A) is a member of the PLA2 superfamily that catalyses the cleavage of fatty acids from the sn-2 position of phospholipids. PLA2 isoenzymes vary in their cellular localizations, Ca2+ sensitivities and substrate specificities. cPLA2 mRNA is widely expressed in tissues and in various cell types including platelets, macrophages and endothelial cells.

Application

Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Cytosolic phospholipase A2 (cPLA2) catalyse the synthesis of precursors of proinflammatory mediators, such as prostaglandins and leukotrienes, through the release of arachidonic acid (AA) from membrane phospholipids. PLA2s play crucial roles in several cellular processes, including intracellular membrane trafficking, differentiation, proliferation and apoptosis. They are thought to play a role in oxidative and inflammatory responses in cerebral ischemia, Alzheimer′s disease (AD) and neuronal injury. Upon cell stimulation, cPLA2 is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol to the endoplasmic reticulum and the nuclear membrane. cPLA2 is phosphorylated and activated by either ERK1/2, p38 MAPK or JNK at three sites, Ser505, Ser515 and Ser727, depending on the cell type and agonist.

Linkage

synthetic peptide corresponding to amino acids 242-258 of human cPLA2 conjugated to KLH. The corresponding sequence is identical in mouse and rat cPLA2

Physical form

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cellular regulation and proposed biological functions of group VIA calcium-independent phospholipase A2 in activated cells
Balsinde J and Balboa MA
Cellular Signalling, 17(9), 1052-1062 (2005)
Brain cytosolic phospholipase A2: localization, role, and involvement in neurological diseases
Farooqui AA, et al.
The Neuroscientist : A Review Journal Bringing Neurobiology, Neurology and Psychiatry, 6(3), 169-180 (2000)
Ganggang Wang et al.
Cell death & disease, 15(8), 573-573 (2024-08-09)
Hepatocellular carcinoma (HCC) is a common malignant tumor with a complex immune evasion mechanism posing a challenge to treatment. The role of the S100A10 gene in various cancers has garnered significant attention. This study aims to elucidate the impact of

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