Solutions of this product are not sterile and contain a preservative, ProClin, to control contamination. In theory you could sterile filter P8920 through low protein binding filters, such as PVDF or PES. Sterile filtering will not remove the ProClin from P8920. Live cells should not be exposed to ProClin, as ProClin interrupts the Krebs cycle at 4 different sites. It would be best to purchase the powder as P1524 and then sterile filter after the solution has been prepared.
P8920
Poly-L-lysine solution
Poly-L-lysine solution
1 of 2 reviewers received a sample product or took part in a promotion
0.1 % (w/v) in H2O
Synonym(s):
2-(4-Cyanoanilino)-4,6-dichloropyrimidine, 4-[(4,6-Dichloro-2-pyrimidinyl)amino]benzonitrile
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Pricing and availability is not currently available.
Recommended Products
biological source
synthetic
shelf life
Expiry date on the label.
mol wt
150,000-300,000
concentration
0.1 % (w/v) in H2O
color
colorless
application(s)
hematology
histology
storage temp.
room temp
SMILES string
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key
WBSCNDJQPKSPII-KKUMJFAQSA-N
Looking for similar products? Visit Product Comparison Guide
Application
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.
Biochem/physiol Actions
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
Components
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Preparation Note
This product is supplied as a 0.10% (w/v) solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.
replaced by
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Kimberly Cramer et al.
International journal of molecular sciences, 20(14) (2019-07-13)
In situ visualization of molecular assemblies near their macromolecular scale is a powerful tool to investigate fundamental cellular processes. Super-resolution light microscopies (SRM) overcome the diffraction limit and allow researchers to investigate molecular arrangements at the nanoscale. However, in bacterial
Expression of the receptor activator for nuclear factor-?B ligand and osteoprotegerin in chronic otitis media.
Kuczkowski J et al.
American Journal of Otolaryngology, 31, 404-404 (2010)
Distinctive expression of chemokines and transforming growth factor-beta signaling in human arterial endothelium during atherosclerosis.
Volger OL, et al.
The American Journal of Pathology, 171, 326-337 (2007)
Hermann C Altmeppen et al.
Molecular neurodegeneration, 6, 36-36 (2011-05-31)
The cellular prion protein (PrPC) fulfils several yet not completely understood physiological functions. Apart from these functions, it has the ability to misfold into a pathogenic scrapie form (PrPSc) leading to fatal transmissible spongiform encephalopathies. Proteolytic processing of PrPC generates
Guojun Shi et al.
The Journal of clinical investigation, 127(10), 3897-3912 (2017-09-19)
Peptide hormones are crucial regulators of many aspects of human physiology. Mutations that alter these signaling peptides are associated with physiological imbalances that underlie diseases. However, the conformational maturation of peptide hormone precursors (prohormones) in the ER remains largely unexplored.
Articles
Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.
Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.
Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.
Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.
Related Content
Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.
Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.
Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.
Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.
-
Bonjour, Est ce que cette PLL est stérile ? Est-il possible de la filtrer ? Je vous remercie. Cordialement. Mme Boroni
1 answer-
Helpful?
-
-
Hello! How many microscopy slides can I adhesive for every 100 milliliters of this solution( Poli-L- Lisine),? Gratitude!
1 answer-
The number of slides that may be coated is dependent upon the surface area. The general use instructions recommend using 0.5 - 1.0 mL per 25 cm2 coating area - the 25 cm2 value is based on a T-25 tissue culture flask. A standard microscope slide has an approximate area of 1.3 cm2.
Please see the link below to review the product datasheet which included a general protocol for coating: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/784/704/p6407pis.pdf
Helpful?
-
-
Is Product P8920, Poly-L-lysine solution, recommended for cell culture use?
1 answer-
No, the product contain 0.01% thimerosal as a preservative. For cell culture use, product P4832 is recommended. This solution has the same molecular weight as P8920 but contains no preservative. P4832 is sterile filtered and is considered suitable for cell culture use.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What are the recommended uses for Product P8920, Poly-L-lysine solution?
1 answer-
P8920 was developed for use in coating slides intended for use in either histology or immunohistochemistry. Poly-L-lysine is preferred for uses such as silver staining procedures that require an alkaline pH. Silane coated slides may be more effective is a low pH is required.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
