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MAK072

Sigma-Aldrich

Pyruvate Kinase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

hematological disorder; cancer

storage temp.

−20°C

General description

Pyruvate kinase (PK) is an enzyme that catalyzes the final step of glycolysis, the transfer of a phosphate group from phospho(enol)pyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, due to defects in pyruvate kinase expression or activity, is the second most common cause of hemolytic anemia.

The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.

Application

Pyruvate Kinase Activity Assay Kit has been used to determine the activity of pyruvate kinase in samples.

Suitability

Suitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc

Principle

The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334,H412

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Therapeutic and Biochemical Effects of Garlic (Allium sativum) on Acrylamide Toxicity in Rabbits: Glycolytic Pathway.
Elmahdi B, et al.
Int. J. Pharmacol., 12(4), 429-434 (2016)
Over expression of hyaluronan promotes progression of HCC via CD44-mediated pyruvate kinase M2 nuclear translocation.
L Jing-Huan, et al.
American Journal of Cancer Research, 6(2), 509-509 (2016)
Boa Kim et al.
The Journal of clinical investigation, 128(10), 4543-4556 (2018-09-18)
The M2 isoform of pyruvate kinase (PKM2) is highly expressed in most cancer cells, and has been studied extensively as a driver of oncogenic metabolism. In contrast, the role of PKM2 in nontransformed cells is little studied, and nearly nothing
Allison Lesher Williams et al.
Physiological genomics, 50(7), 479-494 (2018-04-14)
Alternative splicing of RNA is an underexplored area of transcriptional response. We expect that early changes in alternatively spliced genes may be important for responses to cardiac injury. Hypoxia inducible factor 1 (HIF1) is a key transcription factor that rapidly
HIF1α modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1--3 and PKM2.
Prigione A, et al.
Stem Cells, 32(2), 364-376 (2014)
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