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ISO2

Sigma-Aldrich

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Synonym(s):

Mouse Antibody Isotyping Kit

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About This Item

MDL number:
MFCD08705607
UNSPSC Code:
12352203
NACRES:
NA.32

usage

sufficient for 1000 tests (clones) (by ELISA)
 sufficient for 40 tests (clones) (by immunodiffusion, ODD)

shipped in

wet ice

storage temp.

2-8°C

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General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Application

Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Features and Benefits

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

Kit Components Only

Product No.
Description
  • goat antisera to mouse IgA .2 mL
  • goat antisera to mouse IgG3 .2 mL
  • goat antisera to mouse IgG1 .2 mL
  • goat antisera to mouse IgG2a .2 mL
  • goat antisera to mouse IgG2b .2 mL
  • goat antisera to mouse IgM .2 mL

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yuan Dong et al.
SLAS discovery : advancing life sciences R & D, 25(3), 310-319 (2019-09-29)
D-dimer is an essential diagnostic index of thrombotic diseases. Since the existing anti-D-dimer antibodies vary in quality and specificity, a search for alternative anti-D-dimer antibodies is required. The present study aimed to screen a novel monoclonal antibody (mAb) against D-dimer
Qimin Wang et al.
Frontiers in microbiology, 11, 1961-1961 (2020-09-15)
The extracellular domain of influenza M2 protein (M2e) is highly conserved and is a promising target for development of universal influenza vaccines. Here, we synthesized a peptide vaccine consisting of M2e epitope linked to a fibrillizing peptide, which could self-assemble
Generation and characterization of novel stromal specific antibodies
Halder S, et al.
Cell Research, 15(9), 739-739 (2005)
Masanori Onda et al.
Journal of immunology (Baltimore, Md. : 1950), 177(12), 8822-8834 (2006-12-05)
Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell
Manu Kurian Mathew et al.
The Journal of veterinary medical science, 81(12), 1753-1762 (2019-10-28)
Equine influenza is a leading cause for respiratory illness in equines. Major control measures involve vaccination which requires continuous harmonization owing to antigenic drift. The present study focused on assessing the protective efficacy of an inactivated recombinant equine influenza virus
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Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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