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I5271

Sigma-Aldrich

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IL-1β, Lymphocyte activating factor

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Interleukin-1β from mouse, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤0.010 ng/mL ED50

quality

endotoxin tested

mol wt

17.5 kDa (containing 153 amino acid residues)

packaging

pkg of 5X5 μg
pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤0.1ng/mg

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Il1b(16176)

General description

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.

Application

Interleukin-1β from mouse has been used:
  • as a proinflammatory agent, to induce inflammatory signaling cascades mediated cellular damage
  • to study its effect on fetuin A-induced inflammatory response in podocyte
  • to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes

Biochem/physiol Actions

Interleukin-1β (IL-1β) is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

Physical form

Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0

Analysis Note

The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells.

Other Notes

The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of interleukin-1 beta as a key mediator in the upregulation of Cav3. 2-USP5 interactions in the pain pathway
Stemkowski PL, et al.
Molecular Pain, 13 (2017)
Mesenchymal stem cells stimulate protective genetic reprogramming of injured cardiac ventricular myocytes
Rogers TB, et al.
Journal of Molecular and Cellular Cardiology, 50(2), 346-356 (2011)
Wuttichai Jaidee et al.
Journal of natural products, 82(2), 283-292 (2019-01-30)
Four new chalcones (1, 10, 13, and 14), a new flavanone, (9), a new amide (8), and 19 known compounds were acquired from Melodorum siamensis. The structures were established by NMR and MS data analyses. Compounds 1 (er 1.4:1) and
TNFalpha stimulates Map4k4 expression through TNFalpha receptor 1 signaling to cJUN and ATF2
Tesz GJ, et al.
Test (2007)
TNFalpha stimulates Map4k4 expression through TNFalpha receptor 1 signaling to cJUN and ATF2
Tesz GJ, et al.
The Journal of Biological Chemistry (2007)

Articles

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

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