The molecular weight of this enzyme has not been determined. The chain structure, number of sulfide bonds, and other parameters have not been characterized. However, a similar recombinant version, product G0660, lists a molecular weight of approximately 68.5 kDa. Other sources report the molecular weight of a-Glucosidase from Saccharomyces cerevisiae at approximately 63 kDa.
G5003
α-Glucosidase from Saccharomyces cerevisiae
Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
Synonym(s):
α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
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About This Item
Pricing and availability is not currently available.
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type
Type I
Quality Level
form
lyophilized powder
specific activity
≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
contains
phosphate buffer salts and EDTA as balance
composition
Protein, ≥50%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
foreign activity
β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
greener alternative category
, Enabling
relevant disease(s)
diabetes (management of non-insulin-dependent diabetes mellitus)
storage temp.
−20°C
Gene Information
bakers yeast ... MAL12(853209) , MAL32(852602)
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.
Application
α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.[1]
For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters
Biochem/physiol Actions
α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus[2].
Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.
Packaging
Sold on basis of p-nitrophenyl α-D-glucoside units.
Unit Definition
One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.
Analysis Note
Protein determined by biuret.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
inhibitor
substrate
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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What is the molecular mass of the protein ? More information about the chain structure, number of sulfide bonds, or conditions required for its stability (ions)?
1 answer-
Helpful?
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What is the best method to store a solution of this enzym? Which solvent and wat which temperature? How long can you store it without losing enzymatic activity?
1 answer-
Stock solutions of this enzyme may be aliquoted and stored at -20°C. It is recommended to use within 3 to 6 months.
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