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conjugate
CY3 conjugate
form
buffered aqueous solution
extent of labeling
3-9 mol Cy3 per mol protein
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
wet ice
storage temp.
2-8°C
Application
ExtrAvidin®-Cy3™ is suitable for the following applications:
- Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
- Immunocytochemistry.
- Used for labeling.
Biochem/physiol Actions
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin® is covalently conjugated to Cy3 dye and is used for various techniques.
Physical form
Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Affinity purified protein
Legal Information
Cy3 is a trademark of Cytiva
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A Signore et al.
Diabetologia, 40(12), 1476-1479 (1998-02-03)
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Proceedings of the National Academy of Sciences of the United States of America, 99(5), 3246-3251 (2002-02-28)
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