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DMEM - low glucose

Name: DMEM - low glucose
Brand: SIGMA

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217342

Pricing and availability is not currently available.

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Properties

Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

human osteosarcoma cells and HeLa cells[1]
primary human mesenchymal stromal cells (hBMSCs)[2]
human embryonic kidney cell lines[3]

Preparation Note

Supplement with 0.584 g/L glutamine.

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This Item	D5921	D2429	D6046
Sigma-Aldrich D5546 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D5921 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D2429 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D6046 Dulbecco′s Modified Eagle′s Medium - low glucose
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
components NaHCO₃: yes phenol red: yes glucose: low HEPES: no sodium pyruvate: yes L-glutamine: no	components phenol red: no sodium pyruvate: no glucose: low HEPES: no L-glutamine: no NaHCO₃: yes	components NaHCO₃: no sodium pyruvate: yes phenol red: yes glucose: low L-glutamine: no HEPES: no	components L-glutamine: yes sodium pyruvate: yes phenol red: yes NaHCO₃: yes HEPES: no glucose: low
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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