Recommended Products
![Benzo[a]pyrene solution certified reference material, TraceCERT®, 1000 μg/mL in acetone](/deepweb/assets/sigmaaldrich/product/structures/253/820/be96d879-1811-46c0-8f11-612019691c2d/640/be96d879-1811-46c0-8f11-612019691c2d.png)
biological source
Human x Mouse Hybridoma hybrid
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Human hybrid myeloma
Cell Line Description
Derived by a fusion of NS-1 - Ag4 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes.
Application
Fusion partner and hybridisation studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 1 x 105 to 1 x 106 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service