C8274
Cytohelicase from Helix pomatia
lyophilized powder
Synonym(s):
Snail gut enzyme
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About This Item
Application
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.
Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
Cytohelicase from Helix pomatia has been used:
- in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
- in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
- in the enzyme mix for the analysis of mitosis in anthers
Biochem/physiol Actions
Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.
Analysis Note
Contains glucanase/laminarinase activity
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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