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C6805

Sigma-Aldrich

Monoclonal Anti-Collagen, Type VII antibody produced in mouse

clone LH7.2, ascites fluid

Synonym(s):

Anti-EBD1, Anti-EBDCT, Anti-EBR1, Anti-NDNC8

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About This Item

MDL number:
MFCD00164570
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LH7.2, monoclonal

contains

15 mM sodium azide

species reactivity

human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine

technique(s)

dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections
western blot: suitable

isotype

IgG1

UniProt accession no.

Q02388

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL7A1(1294)

General description

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type VII is the crucial component of anchoring fibrils and mutation in type VII collagen gene (COL7A1) leads to epidermolysis bullosa dystrophica. Monoclonal anti-collagen, type VII antibody can be used to distinguish invasive melanoma from non-invasive melanoma by visualizing the appearance and integrity of epidermal basement membrane. It can also be used in immunoblotting. Mouse anti-collagen type VII antibody reacts specifically with an epitope located on collagenase digested type VII collagen (150 kDa). The product also reacts with the basement membrane zone of stratified squamous epithelia of sheep, pig, bovine, guinea pig, goat and human.
Collagen type VII alpha 1 chain (COL7A1) is majorly expressed in skin, secreted by keratinocytes and fibroblasts. The gene is located on human chromosome 3p21.31.

Immunogen

insoluble fractions of neonatal human foreskin epidermal cells

Application

Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in immuno-gold labelling.
Monoclonal anti-collagen type VII antibody can be used as a primary antibody in virus titration. It can also be used for the localization of type VII collagen in different immunochemical assays like dot blot, immunocytochemistry and ELISA.

Biochem/physiol Actions

Collagen type VII alpha 1 chain (COL7A1) is required for epithelium-to-stroma anchorage in skin, mucosa and cornea. It is considered as an immediate-early response gene for transforming growth factor β (TGF-β)/SMAD (mothers against decapentaplegic) signaling pathway.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000424120
0000424116
0000424116
0000424120
0000386044

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Type VII Collagen in the Human Accommodation System: Expression in Ciliary Body, Zonules, and Lens Capsule
Wullink B, et al.
Investigative Ophthalmology & Visual Science, 59(2), 1075-1083 (2018)
Molecular basis of dystrophic epidermolysis bullosa: mutations in the type VII collagen gene (COL7A1).
Jarvikallio A, Pulkkinen L
Human Mutation, 10(5), 338-347 (1997)
Type VII collagen associated with the basement membrane of amniotic epithelium forms giant anchoring rivets which penetrate a massive lamina reticularis
Ockleford CD, et al.
Placenta, 34(9), 727-737 (2013)
Martin A Barbier et al.
Current protocols, 2(1), e353-e353 (2022-01-28)
Efficient gene transfer into cultured fibroblasts and keratinocytes during retroviral transduction is a critical step toward the treatment of genodermatoses such as epidermolysis bullosa. However, achieving high transduction rates is still a difficult task, particularly for the insertion of large
SMAD3/4-dependent transcriptional activation of the human type VII collagen gene (COL7A1) promoter by transforming growth factor ?
Vindevoghel L, et al.
Proceedings of the National Academy of Sciences of the USA, 95(25), 14769-14774 (1998)
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Questions

  1. I would like to know the concentration of this antibody.

    1 answer

    1. This product is a mouse ascites fluid. The concentration may range from 1.5 - 3.0 mg/mL and is reported in the lot specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/c6805#product-documentation

      Please see the following link to review additional information available in the product Datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/886/329/c6805dat.pdf

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