A0705

Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Rabbit Anti-Bovine IgG (whole molecule)−AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162168
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: DB, ELISA (d)
• Citations: 17

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: bovine
• technique(s): direct ELISA: 1:30,000, dot blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulins (Igs) belongs to immunoglobulin super-family (IgSF). Igs in general, consist of two heavy (H) chains and two light (L) chains. The L chain can be either κ or a λ chain. Each chain contains one NH2-terminal “variable" (V) region and one or more COOH-terminal “constant” (C) regions. Each of which consists of two sandwiched β pleated sheets ′pinned′ together by a disulfide bridge. There are five main classes of Igs based on heavy chain C domains, they are, IgM, IgG, IgA, IgD, and IgE isotypes. IgG can be divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. Each subclass possesses distinct biologic properties.

Immunogen

Purified bovine IgG

Application

Anti-Bovine IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in

• enzyme linked immunosorbent assay (ELISA)
• development of immunosensors
• western blot analysis

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin [1]. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration [2].

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)