24340C
EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells
protein free, dry powder, suitable for cell culture
Pharma Manufacturing
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description
for research or for further manufacturing use
without L-glutamine
Quality Level
form
dry powder
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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General description
EX-CELL® 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System™ and DHFR- selection systems).
Quantity
Formulated to contain 21.0 grams of powder per liter of medium.
Reconstitution
Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.
Legal Information
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
GS System is a trademark of Lonza Group Ltd.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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