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Sigma-Aldrich

Methyl Green

zinc chloride salt, <0.5% crystal violet, Dye content 80 %

Synonym(s):

Ethyl Green

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About This Item

Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
Colour Index Number:
42590
Beilstein:
3914106
EC Number:
230-415-4
MDL number:
MFCD00151094
UNSPSC Code:
12171500
PubChem Substance ID:
24859418
NACRES:
NA.47

Assay

>75.0% (HPLC)

form

solid

composition

Dye content, 80%

impurities

<0.5% crystal violet

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 1 mg/mL

λmax

420 nm (2nd)
635 nm

ε (extinction coefficient)

≥14000 at 311-317 in water at 0.005 g/L
≥50000 at 629-635 in water at 0.005 g/L
≥9000 at 420-426 in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

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General description

Methyl Green is a triphenylmethane dye which is mainly used in combination with pyronin. It is suitable for the detection of DNA and RNA.

Application

Used in the Methyl Green-Pyronin methods to demonstrate DNA and RNA in tissue sections.

Other Notes

Zinc chloride salt

Signal Word

Danger

Hazard Statements

H314,H335,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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