T2928

Anti-Tyrosine Hydroxylase Antibody

mouse monoclonal, TH-16

• Synonym(s): Anti-DYT14, Anti-DYT5b, Anti-TYH
• MDL number: MFCD00213051
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• product name: Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse, clone TH-16, ascites fluid
• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: TH-16, monoclonal
• contains: 15 mM sodium azide
• species reactivity: rat, sheep, human, monkey, bovine, rabbit, guinea pig
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable using native or SDS-denatured TH.; microarray: suitable; western blot: 1:8,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
• isotype: IgG1
• UniProt accession no.: P07101
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TH(7054) ; rat ... Th(25085)

Description

General description

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms.

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH₄) and O₂ as cosubstrates, and Fe²⁺ as a cofactor.

Specificity

The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis.

Immunogen

rat tyrosine hydroxylase (TH).

Application

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in

• western blotting
• immunofluorescence labelling
• immunohistochemical staining
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling.

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable