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Key Documents

SRP6267

Sigma-Aldrich

C-reactive/CRP human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

C-reactive protein, CRP, PTX1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 24 kDa
observed mol wt 26 kDa (DTT-reduced. Phe17 is the predicted N-terminus.)

packaging

pkg of 100 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P02741

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CRP(1401)

General description

C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat, respectively.

Biochem/physiol Actions

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yeongseok Jang et al.
Nanoscale, 12(18), 9980-9990 (2020-03-05)
We present multiple-bent multi-walled carbon nanotubes (MWCNTs) that enable the picomolar detection of C-reactive protein (CRP), which is considered to be a promising biomarker for various diseases. The MWCNTs were grown via chemical vapor deposition repeating the asymmetric catalytic CNT
C-reactive protein attenuates endothelial progenitor cell survival, differentiation, and function: further evidence of a mechanistic link between C-reactive protein and cardiovascular disease.
Verma S
Circulation, 109, 2058-2067 (2004)
Human C-reactive protein: expression, structure, and function.
Volanakis JE
Molecular Immunology, 38, 189-197 (2001)
Function of C-reactive protein
Du Clos TW
Annals of Medicine, 32, 274-278 (2000)

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