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SHC001V

Sigma-Aldrich

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles

Contains no shRNA insert

Synonym(s):

MISSION®, MISSION® Control Transduction Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

Quality Level

100
200

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

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General description

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

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SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC004V

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

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MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, Targets no known genes from any species

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (-)-Gossypol (AT-101).
Mani J
BMC Cancer, 15, 224-224 (2015)
Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.
Miura N
Oncotarget (2016)
Deepak Parashar et al.
Cancer research, 80(24), 5554-5568 (2020-10-23)
Peritoneal spread is the primary mechanism of metastasis of ovarian cancer, and survival of ovarian cancer cells in the peritoneal cavity as nonadherent spheroids and their adherence to the mesothelium of distant organs lead to cancer progression, metastasis, and mortality.
Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor
Nature, 099655-099655 (2017)
Xiaobo Luo et al.
The Journal of clinical investigation, 130(4), 1635-1652 (2019-12-25)
The incidence of human papillomavirus-positive (HPV+) head and neck squamous cell carcinoma (HNSCC) has surpassed that of cervical cancer and is projected to increase rapidly until 2060. The coevolution of HPV with transforming epithelial cells leads to the shutdown of
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Articles

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Protocols

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

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