S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

• Synonym(s): Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Empirical Formula (Hill Notation): C₂₀H₁₉ClN₄
• CAS Number: 477-73-6
• Molecular Weight: 350.84
• Colour Index Number: 50240
• Beilstein: 3924099
• EC Number: 207-518-8
• MDL number: MFCD00011759
• UNSPSC Code: 12171500
• PubChem Substance ID: 24899820
• NACRES: NA.47

Properties

• product name: Safranin O, certified by the Biological Stain Commission
• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥80%
• technique(s): microbe id | staining: suitable
• color: green to very dark green
• solubility: H₂O: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• InChI key: OARRHUQTFTUEOS-UHFFFAOYSA-N

Description

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Application

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Suitability

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H318
• Precautionary Statements: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves