S8820

Protease Inhibitor Cocktail

SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use

• Synonym(s): Protease Inhibitor Tablets, protease inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: SIGMAFAST^™ Protease Inhibitor Tablets, For General Use
• biological source: bovine lung; bovine milk
• Quality Level: 200
• form: tablet
• solubility: water: 1 tablet/100mL, clear to hazy, colorless
• storage temp.: 2-8°C

Description

General description

SIGMAFAST^™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.

The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select^® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

SIGMAFAST Protease Inhibitor Tablets has been used:

• to wash raw semen for membrane protein analysis
• in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
• as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
• as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Features and Benefits

• Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
• Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
• Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
• Tested and optimized for general use: proven performance and reliability for a wide range of applications.

Components

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Quantity

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H314,H335
• Precautionary Statements: P260 - P271 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves