Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

RAB0436

Sigma-Aldrich

Human Osteopontin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

OPN

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SPP1(6696)

Related Categories

General description

The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human OPN

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Osteopontin ELISA Kit has been used in the quantification of serum osteopontin (OPN) in human blood samples.

Biochem/physiol Actions

Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Octavio Gamaliel Aztatzi-Aguilar et al.
PloS one, 16(3), e0249057-e0249057 (2021-03-26)
Osteopontin (OPN) is a protein involved in inflammatory illnesses such as fibrosis and cancer; its overexpression in cardiovascular diseases promotes the biomineralization of blood vessels and other soft tissues. Moreover, there is an active component of oxidative stress related with
EFFECT OF MITRAL VALVE SURGERY FOR MITRAL REGURGITATION ON SERUM OSTEOPONTIN LEVELS
Seddik M, et al.
Al-Azhar Assiut Medical Journal, 13(3), 1-1 (2015)
Can Osteopontin Be Considered a Biomarker for Endometriosis?
Albu D R, et al.
Revista de Chimie (Bucharest, Romania), 13(3), 1-1 (2017)
Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation.
Murugaiyan G, et al.
Proceedings of the National Academy of Sciences of the USA, 107, 11495-11500 (2010)
Osteopontin, a protein with cytokine-like properties, is associated with inflammation in Crohn's disease.
Agnholt J, et al.
Scandinavian Journal of Immunology, 65, 453-460 (2007)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service