Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

R1145

SAFC

RPMI-1640 Medium

Without ʟ-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonym(s):

RPMI Medium, RPMI-1640 Culture Medium, Roswell Park Memorial Institute 1640 medium

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

product name

RPMI-1640 Medium, 10 ×, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Reconstitution

Supplement with 0.3 g/L L-glutamine, 2.0 g/L sodium bicarbonate, 0.001 g/L folic acid at 1×.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Tamara Veiga-Parga et al.
PLoS pathogens, 7(12), e1002427-e1002427 (2011-12-17)
Ocular herpes simplex virus infection can cause a blinding CD4⁺ T cell orchestrated immuno-inflammatory lesion in the cornea called Stromal Keratitis (SK). A key to controlling the severity of SK lesions is to suppress the activity of T cells that
Ryoichi Arita et al.
Diabetes, 58(1), 215-226 (2008-10-09)
Leukocyte adhesion in retinal microvasuculature substantially contributes to diabetic retinopathy. Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated. Localization of RhoA/ROCK and Rho activity were examined in
Fhernanda R Smiderle et al.
BMC complementary and alternative medicine, 11, 58-58 (2011-07-27)
Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. Crude mushroom extracts have been tested without detailed chemical analyses of its polysaccharide content. For the
Yoichi Matsuo et al.
International journal of cancer, 124(4), 853-861 (2008-11-28)
CXC-chemokines are involved in the chemotaxis of neutrophils, lymphocytes and monocytes. However, role of these chemokines in tumorigenesis, especially with regard to interaction between tumor and its microenvironment, has not been clearly elucidated. The purpose of this study was to
Ahmed Mosa et al.
Infection and immunity, 77(7), 2908-2918 (2009-04-29)
We analyzed the defensive role of the cytosolic innate recognition receptor nucleotide oligomerization domain 1 (NOD1) during infection with Listeria monocytogenes. Mice lacking NOD1 showed increased susceptibility to systemic intraperitoneal and intravenous infection with high or low doses of L.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service