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PP0201

Sigma-Aldrich

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

Quality Level

200

storage temp.

2-8°C

General description

Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.

Application

GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.
GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.
Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • 10× Reaction Buffer 1 mL/vial

Kit Components Also Available Separately

Product No.
Description
SDS
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDS
  • R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mgSDS
  • O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mgSDS
  • M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mLSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Target Organs

Liver,Heart

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown
Haarmann A, et al.
PLoS ONE, 5(10), e13568-e13568 (2010)
Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from the edible mushroom Schizophyllum commune
Chumkhunthod P, et al.
Biochim. Biophys. Acta Gen. Subj., 1760(3), 326-332 (2006)
Podjana Chumkhunthod et al.
Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
Elena Schwagerus et al.
Pflugers Archiv : European journal of physiology, 467(11), 2257-2273 (2015-02-14)
Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human
Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)
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Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Protocols

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

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