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MITOISO1

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Synonym(s):

Mitochondria Purification Kit

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About This Item

EC Number:
232-650-8
UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

usage

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

shelf life

2 yr

technique(s)

fractionation: suitable

shipped in

dry ice

storage temp.

−20°C

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Application

Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
  • for the isolation of mitochondria from glomeruli and heart
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Features and Benefits

  • Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
  • Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
  • Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
  • Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Kit Components Also Available Separately

Product No.
Description
SDS
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidSDS

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CYTOCOX1

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CS0720

Citrate Synthase Assay Kit, 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

MITOISO2

Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

CS0760

Isolated Mitochondria Staining Kit, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

MITOISO3

Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Chris Stirone et al.
Molecular pharmacology, 68(4), 959-965 (2005-07-05)
We report here that estrogen (E(2)) modulates mitochondrial function in the vasculature. Mitochondrial dysfunction is implicated in the etiology of vascular disease; thus, vasoprotection by estrogen may involve hormonal effects on the mitochondria. To test this hypothesis, mitochondria were isolated
Janna R Jackson et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 66(7), 751-764 (2011-04-02)
This study analyzed the capacity of resveratrol, a naturally occurring polyphenol, to reduce aging-induced oxidative stress and protect against sarcopenia. Middle-aged (18 months) C57/BL6 mice were randomly assigned to receive either a control diet or a diet supplemented with 0.05%
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