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M5257

Sigma-Aldrich

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

Synonym(s):

L-Malate:NADP+ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme

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About This Item

CAS Number:
9028-47-1
Enzyme Commission number:
1.1.1.40 (BRENDA, IUBMB)
EC Number:
232-834-8
MDL number:
MFCD00131568
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

chicken liver

Quality Level

200

form

ammonium sulfate suspension

specific activity

10-30 units/mg protein (modified Warburg-Christian)

storage temp.

2-8°C

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General description

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Application

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Biochem/physiol Actions

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

Unit Definition

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.

Physical form

Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Dietary manganese requirement of juvenile yellow catfish Pelteobagrus fulvidraco, and effects on whole body mineral composition and hepatic intermediary metabolism
Tan, X., et al.
Aquaculture (Amsterdam, Netherlands), 326, 68-73 (2012)
Establishment and biological characterization of fibroblast cell line from the Langshan chicken
Guan, W., et al.
Cell Proliferation, 42, 157-163 (2010)
Synthesis and Degradation of Malic Enzyme in Chick Liver.
Silpananta, P
The Journal of Biological Chemistry, 264, 5754-5761 (1971)
A R Ashton
Archives of biochemistry and biophysics, 345(2), 251-258 (1997-10-06)
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) was purified to near-homogeneity from leaves of the C4 dicot Flaveria bidentis and shown to possess intrinsic NAD-dependent malic enzyme activity. The NAD-dependent activity is optimal at pH 7.5 and in the presence of Mn2+.
K N Rao et al.
Gastroenterology, 113(1), 238-248 (1997-07-01)
We showed previously that the peroxisome proliferators di(2-ethylhexyl)phthalate (DEHP), clofibrate, and 4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyl)acetamide (BR931) alter hepatic sex steroid metabolism and receptor expression during induction of hepatic hyperplasia and hepatocellular carcinoma (HCC) in rats. The aim of this study was
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Instructions for working with enzymes supplied as ammonium sulfate suspensions

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