M0321
MDM2, GST-tagged human
recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥75% (SDS-PAGE)
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
description
contains an N-terminal GST tag
Assay
≥75% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~84 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MDM2(4193)
General description
Murine double minute 2 (MDM2) encoding gene is localized at 12q15 in the human chromosome. It is a multidomain nuclear phosphoprotein. It possesses really interesting new gene (RING) finger domain and its function and structural integrity is required for the action of MDM2. It also has central acidic domain.
Biochem/physiol Actions
Murine double minute 2 (MDM2) is a chief regulator of the p53 tumor suppressor protein. It is a multifunctional oncoprotein that contributes to both p53-dependent and -independent roles in oncogenesis. MDM2 has various p53-independent functions that contribute to its activity as an oncogene.
Overexpression of Murine double minute 2 (MDM2) is observed in various cancer like lung, gastric, colorectal, hepatocellular, breast, endometrial, and prostate. A common single nucleotide polymorphism is observed at 309 amino acid (from T to G) increases the susceptibility to cancer.
MDM2 is a negative regulator of the p53 tumor suppressor.
Mdm2 functions as an E3 ubiquitin ligase and an inhibitor of p53 transcriptional activation.
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.
Preparation Note
Supplied as a truncated version (202-end) with an N-terminal GST tag
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The Mdm2 RING domain C-terminus is required for supramolecular assembly and ubiquitin ligase activity
Poyurovsky MV, et al.
The Embo Journal, 26(1), 90-101 (2007)
MDM2 promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma protein
Sdek P, et al.
Molecular Cell, 20(5), 699-708 (2005)
Fluorescence in situ hybridization for MDM2 gene amplification as a diagnostic tool in lipomatous neoplasms
Weaver J, et al.
Modern Pathology, 21(8), 943-943 (2008)
Association between MDM2 SNP309 and endometrial cancer risk: A PRISMA-compliant meta-analysis
Zou X, et al.
Medicine, 97(49) (2018)
C Dehay et al.
Nature, 331(6154), 348-350 (1988-01-28)
Our understanding of the development of cortical connectivity largely stems from studies of the ontogeny of interhemispheric pathways in carnivores, rodents and lagomorphs. Early in development, cortical neurons projecting to the contralateral hemisphere through the corpus callosum (callosal projection neurons)
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