L9043
Lysostaphin from Staphylococcus simulans
recombinant, expressed in E. coli, lyophilized powder
Synonym(s):
Glycyl-glycine Endopeptidase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
bacterial
Quality Level
recombinant
expressed in E. coli
Assay
≥90% (HPLC)
form
lyophilized powder
specific activity
≥3000 unit/mg solid
technique(s)
molecular cloning: suitable
suitability
suitable for sequence analysis test
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−20°C
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General description
Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C.
Application
Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.
Biochem/physiol Actions
A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening.
The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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L Ramadurai et al.
Journal of bacteriology, 179(11), 3625-3631 (1997-06-01)
A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading
C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
Yang, G.
Acta Biochimica et Biophysica Hungarica, 40, 452-458 (2008)
X Wang et al.
Gene, 102(1), 105-109 (1991-06-15)
The nucleotide (nt) sequence of a 2.0-kb NheI-XbaI DNA fragment containing a peptidoglycan hydrolase-encoding gene, lytA, tentatively identified as encoding an N-acetylmuramyl-L-alanine amidase, from Staphylococcus aureus, was determined. The nt sequencing revealed an open reading frame (ORF) of 1443 bp
Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)
Grégory Francius et al.
Journal of bacteriology, 190(24), 7904-7909 (2008-10-07)
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby
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