L2143

Lipopolysaccharides from Escherichia coli K-235

purified by phenol extraction

• Synonym(s): LPS
• EC Number: 297-473-0
• MDL number: MFCD00164401
• UNSPSC Code: 12352201
• NACRES: NA.25

Properties

• biological source: Escherichia coli (K-235)
• Quality Level: 200
• form: lyophilized powder
• purified by: phenol extraction
• impurities: <3% Protein (Lowry)
• color: white to faint yellow
• solubility: water: soluble
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

This product is phenol extracted from E. coli serotype K235. The source strain is ATCC 13027.

Application

• Establishment of disseminated intravascular coagulation (DIC) model by a single iv administration of Escherichia coli-derived lipopolysaccharide (LPS) to cynomolgus monkeys and evaluation of its pathophysiological status.: This study established a DIC model in cynomolgus monkeys using E. coli-derived LPS, providing insights into the pathophysiological status and potential therapeutic interventions for DIC (Minomo et al., 2017).


• Different bacterial lipopolysaccharides as toxicants and stressors in the shrimp Palaemon elegans.: This research explored the toxic effects of various bacterial LPS, including E. coli K-235, on the shrimp Palaemon elegans, advancing the understanding of LPS-induced stress responses in marine organisms (Lorenzon et al., 2002).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves